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Research Impact:




Associate Professor Dr Crystale Lim Siew Ying

Associate Professor of Molecular & Cell Biology

Dean | Department of Biotechnology

Academic Qualifications

PhD Molecular Medicine
Postgrad Dip (Tertiary Teaching)
BSc (Hons) Biomedical Sciences

Areas of Interest
Antibiotic resistance and biological responses
Tumorigenesis and eukaryotic quorum sensing
Gene expression profiling
Behaviorial genetics in addiction and autism spectrum disorder
Research

Research Grants

  1. Name of grant: Hibah Internal Penelitian Institut Bio Scientia Internasional Indonesia Ta. 2021/2022 [Indonesia International Institute of Life Sciences (I3L) Institutional Development Research funding scheme]
    ?Funder: Indonesia International Institute of Life Sciences (I3L)
    Project Title: Development of Probiotic Food and Supplement: Functionality of Spray-dried Pediococcus acidilactici
    Code: NA
    Durations: 2022-2023
    Role: Co-researcher
    Amount: Rp. 39,995,000
    Status: Ongoing
  2. Name of grant: Fundamental Research Grant Scheme (FRGS) cycle 2021-01
    ?Funder: MOHE
    Project Title: Molecular characterization of CRISPR-Cas elements in methicillin-resistant Staphylococcus aureus (MRSA) and its potential role for early diagnosis of breast infections caused by MRSA.
    Code: FRGS/1/2021/STG01/ÎåÒ»³Ô¹Ï¹ÙÍø/03/1
    Durations: 07/09/2021- 06/09/2024
    Role: Co-principal investigator
    Amount: RM 166,000
    Status: Ongoing
  3. Name of grant: Fundamental Research Grant Scheme (FRGS) cycle 2020-01
    ?Funder: MOHE
    Project Title: Investigation of different immunoglobulin isotypes of a TCR-like single-domain antibody against a Tuberculosis antigen for diagnostics and therapeutics
    Code: FRGS/1/2020/STG01/USM/02/12
    Durations:01/11/2020- 31/10/2023
    Role: Co-researcher
    Amount: RM 182,800
    Status: Ongoing
  4. Name of grant: Fundamental Research Grant Scheme (FRGS) cycle 2020-01
    Funder: MOHE
    Project Title: Molecular Dynamics-based Approach for Elucidating the Inhibition Mechanism of Mycobacterium tuberculosis Cytochrome P450 CYP121, An Anti-Tuberculosis Drug Target
    Code: FRGS/1/2020/STG01/UPM/02/24
    Durations: 01/11/2020- 31/10/2023
    Role: Co-researcher
    Amount: RM 135,400
    Status: Ongoing
  5. Name of grant: Kotra Pharma Sdn Bhd (private sector research fund)
    Funder: Kotra Pharma Sdn Bhd
    Project Title: Isolation And Identification of Antibiotic-Resistant Bacteria and Detection of Major Viruses from Primary Care Patients with Acute Pharyngitis (sore throat) in the Klang Valley
    Code: Proj-In-KOTRA-FAS-001
    Durations: 2017-current
    Role: Lead researcher
    Amount: RM 154,000
    Status: Ongoing
  6. Name of grant: MTSF Science & Technology Research Grant 2015
    Funder: Toray Science Foundation, Japan (international research fund)
    Project Title: Enhanced Laccase Stability through Nanodispersion for Recalcitrant Dye Effluent Treatment
    Code: RA1200 (TML-CA/15.205)
    Durations: 2015-2017
    Role: Co-researcher
    Amount: RM 20,000
    Status: Completed
  7. Name of grant: Pioneer Scientist Incentive Fund 
    Funder: CERVIE, ÎåÒ»³Ô¹Ï¹ÙÍø University (internal research fund)
    Project Title: Manuka propolis as a potential anti-pathogenicity quorum sensing inhibitor of Gram-negative bacteria
    Code: Proj-In-FAS-023
    Durations: 2015-2017
    Role:  Lead researcher
    Amount: RM 25,000
    Status: Ongoing
  8. Name of grant: Bioactive compounds RGS sub-cluster research grant 
    Funder: CERVIE, ÎåÒ»³Ô¹Ï¹ÙÍø University (internal research fund)
    Project Title: Investigations of Termitomyces heimii and Lignosus rhinocerus extracts and laccases for bio-bleaching and quorum-sensing inhibition
    Code: Proj-In-FAS-021
    Durations: 2013-2016
    Role:  Lead researcher
    Amount: RM 133,000
    Status: Completed
  9. Name of grant: Bioactive compounds RGS sub-cluster research grant 
    Funder: Malaysian Cancer Council-National Academy of Sciences (government research fund)
    Project Title: Farnesol, a eukaryotic quorum-sensing molecule, as an inhibitor of HeLa cell proliferation via telomerase deactivation
    Code: FAS-EX-MAKNA-001
    Durations: 2012-2014
    Role:  Lead researcher
    Amount: RM 25,000
    Status: Completed
  10. Name of grant: L¡¯Oreal for Women in Science (FWIS) National Fellowship from L¡¯Oreal Malaysia-UNESCO (private research fund)
    Funder: L¡¯Oreal Malaysia-UNESCO
    Project Title: Morphology switching and quorum sensing in Candida albicans pathogenesis
    Code: N/A
    Durations: 2005-2007
    Role:  PhD researcher
    Amount: RM 15,000
    Status: Completed

Current Research Project 

  1. Development of Probiotic Food and Supplement: Functionality of Spray-dried Pediococcus acidilactici
  2. Molecular characterization of CRISPR-Cas elements in methicillin-resistant Staphylococcus aureus (MRSA) and its potential role for early diagnosis of breast infections caused by MRSA.
  3. Investigation of different immunoglobulin isotypes of a TCR-like single-domain antibody against a Tuberculosis antigen for diagnostics and therapeutics
  4. Molecular Dynamics-based Approach for Elucidating the Inhibition Mechanism of Mycobacterium tuberculosis Cytochrome P450 CYP121, An Anti-Tuberculosis Drug Target
  5. Modulation of Neurite Outgrowth in Human Model Cell Line by Lignosus Rhinocerotis Subcritical Fluid Extract 
  6. G-I-Y Spirulina Media Kit: A Green Approach for Spirulina Cultivation
  7. Antibiotic Resistance in the Community: Study on Primary Care Patients with Acute Pharyngitis (sore throat) in the Klang Valley
  8. Modulation of Cellular Proliferation and Differentiation with Eukaryotic Quorum Sensing Molecules 
  9. Modulation of Biosensor Gene Expression in Vibrio fisheri and Chromobacterium violaceum in Response to Small Molecules

Research Idea

Do you have a research idea? Let's explore one that sparks joy together!

Publications

  1. SA Dass, V Balakrishnan, N Arifin, CSY Lim, F Nordin and GJ Tye (2022). Front. Immunol. 13:1-18 ISSN 1664-3224
  2. A Eswaran, CSY Lim, SM Chye, APK Ling and RY Koh (2021). ASM Sc. J. 16:1-10 ISSN: 1823-6782
  3. DS Chin, CSY Lim, F Nordin, N Arifin, TG Jun (2021). Curr Pharm Biotechnol 23:552-578 lSSN: 1873-4316
  4. EU Camillius, CJ Chan, CSY Lim, RLH Lim (2021). Applied Microbiology and Biotechnology 105: 2799¨C2813 ISSN: 1432-0614
  5. YH Tan, CSY Lim, KH Wong, V Sabaratnam (2021). International Journal of Medicinal Mushroom 23(6) 1-11
  6. CSC Chong, ZM Bo, WK Tan, BLH Sim, CKC Lee, RLH Lim, CSY Lim (2021). Antibiotic Resistance Patterns of WHO Priority Pathogens Isolated from Acute Pharyngitis Patients in Private Primary Care Clinics. AsPac J. Mol. Biol. Biotechnol. 2021 Vol. 29 (2) Suppl. pg65
  7. N Ismail, YH Tan, RLH Lim, SR Yusof, CSY Lim (2020). Conference abstract in European Journal of Neurology, 27 (Suppl. 1)
  8. CSC Chong, ZM Bo, WK Tan, S Parmasivam, ST Pang, BLH Sim, CKC Lee, RLH Lim, CSY Lim (2020). Asia Pacific Journal of Molecular Biology & Biotechnology 28 (3):52-58
  9. WZ Gan, V Ramachandran, CSY Lim, RY Koh (2019). Journal of Basic and Clinical Physiology and Pharmacology. 31:1-21
  10. TJ Ng, MYM Teo, DS Liew, PE Effiong, JS Hwang, CSY Lim, LLA In (2019). Peer J:e6639
  11. JY Loh, HK Chan, HC Yam, LLA In, CSY Lim (2019). Aquaculture International
  12. WW Chong, CSY Lim, KS Lai JY Loh. In vitro antimicrobial assessment on lactic acid bacteria isolated from common freshwater fishes (2019) Asia-Pacific Journal of Molecular Biology and Biotechnology 27(2):18-25
  13. HS Khoo, N Ismail, L Joko, WK Tan, KK Woo, CSY Lim, YH Tan. Characterisation and decolorisation capacity of laccase from Termitomyces heimii 2019) Malaysian Journal of Biochemistry & Molecular Biology 1:110-117 
  14. CSY Lim (2018). Conference abstract in Journal of Bacteriology & Parasitology
  15. UC Ejike, ZM Bo, CSY Lim, RLH Lim. Antibiotics resistance of commensal throat bacteria from healthy subjects. (2018) Malaysian Journal of Biochemistry & Molecular Biology 1:1-8
  16. Y Tan, CSY Lim, K Wong and V Sabaratnam (2016). Conference abstract in Front. Cell. Neurosci. Conf. Abs: 14th Meeting of the Asian-Pacific Society for Neurochemistry.
  17. YP Tan, ECW Chan, CSY Lim. Potent quorum sensing inhibition by methyl gallate isolated from leaves of Anacardium occidentale L. (cashew). (2015) Chiang Mai Journal of Science 42(3):650-656
  18. PP Chong, CH Tung, NA Abdul Rahman, M Yajima, FW Chin, CSY Lim, ES Go, CML Chan, N Yawata, N Yamamoto. Prevalence and viral load of oncogenic human papillomavirus (HPV) in pterygia in multi-ethnic patients in the Malay Peninsula. (2014) Acta Opthalmologica Doi:10.1111/aos.12427
  19. AT Gemiarto, EWC Chan, CSY Lim (2013). International Journal of Antimicrobial Agents 42S2:S108
  20. SS Chhabbra, PP Chong, KP Ng, CSY Lim (2012). Genetic relatedness of Candida albicans clinical isolates from Candida bloodstream infections in a Malaysian population. Conference abstract in International Journal of Infectious Diseases 16S1: 49.006 (e320).
  21. CSY Lim, Rozita R, HF Seow, PP Chong. Candida and invasive candidiasis: Back to basics. (2012) European Journal of Clinical Microbiology & Infectious Diseases. 31:21-3
  22. CSY Lim, YH Tan, Rozita R, HF Seow, PP Chong (2010). Transcriptome profile of the human endothelial response to high- and low-density infections of Candida albicans. Conference abstract in International Journal of Infectious Diseases. 14S1: 73.014 (e341).
  23. CSY Lim, WF Wong, CH Tung, R Rosli, HF Seow, PP Chong (2008). Differential expression study of a potentially novel gene in Candida albicans morphology switching. International Journal of Infectious Diseases 13S1: 45.032 (e289)
  24. CH Tung, CSY Lim, Syahril A, Rozita R, PP Chong. The effects of different culture media on the cellular morphologies of Candida albicans under hyphal-induction conditions. (2008) Malaysian Journal of Microscopy. 4:1-6
 

Conferences

  1. EduTECH Malaysia 2022, 9-10 March 2022, Virtual. Special invited speaker for Show & Tell. Title: "Engaging students in a face-to-face classroom ¨C beyond the 'normal' lecture"
  2. EduTECH Asia 2021, 9-11 Nov 2021, Virtual. Invited speaker for Show & Tell. Title: "Engaging students in a face-to-face classroom ¨C beyond the 'normal' lecture"
  3. EduTECH Asia 2020, 30 Oct ¨C 6 Nov 2020, Virtual. Invited speaker for Townhall Meeting, 4 Nov 2020. Title: ¡°Best practices for engaging students with hybrid learning models¡±
  4. EduTECH Asia 2019, 4-6 Nov 2019, Suntec City Convention Center, Singapore. Invited speaker: "Teachtechtalk" Title: ¡° From Sci-Fi to Sci-Fact: Life Science is the 5th Industrial Revolution¡±
  5. 29th Malaysian Society of Neuroscience (MSN) Annual Scientific Meeting, Kota Bharu, Kelantan. Invited speaker Title: ¡°Extracts of Tiger Milk Mushroom Attenuate Hydrogen Peroxide- and Amyloid Beta-induced Neurotoxicity in Human Neuroblastoma Cell Line SH-SY5Y.¡±
  6. Education Investor Global Asia Summit 2019, Marina Bay Sands, Singapore. Invited panelist. Title: ¡°Overview of EdTech in ASEAN countries¡±
  7. Infectious Diseases Forum 2018, 28 Oct 2018, Doubletree by Hilton, KL. Invited speaker. Title: ¡°Strep throat or sore throat: Antibiotic appropriateness among general practitioners in the Klang Valley¡±
  8. EduTECH Asia 2018, 8-10 Oct 2018, Suntec City Convention Center, Singapore. Workshop trainer. Title: ¡°Gamification for Higher Education¡± & Tertiary Leaders Forum Discussion Panellist. Title: ¡°Making a case for experiential learning in higher education¡±
  9. 7th International Conference on Bacteriology &  Infectious Diseases, 4-5 Jun 2018, Osaka, Japan. Keynote speaker. Title: ¡°Strep throat or sore throat: Towards good antibiotic appropriateness practices among general practitioners¡±
  10. 5th Annual NGS & Single Cell Analysis Asia Congress 2017, Singapore. Invited speaker. Title: ¡°Antibiotic resistance & the microbiome: Microbiome species identification platform wars-The challenge of anything & everything when you have nearly next to nothing¡±
  11. UTAR Symposium on Science Education 2017, UTAR, Selangor. MTSF-Toray Invited speaker. Title: ¡°Back to basics - Making science fun again: bridging high school and university¡±
  12. Inaugural Faculty of Medicine & Health Sciences Scientific Meeting 2017, UTAR, Selangor. Invited speaker. Title: ¡°Major respiratory viruses associated with sore throats: Reducing the pain of viral load determination via real-time PCR¡±
  13. Infectious Diseases Forum 2017, Equatorial Hotel, Penang. Invited speaker. Title: ¡°Antibiotics and sore throats: Major viruses and antibiotic resistance of bacteria isolated from primary care patients with acute pharyngitis (sore throat) in the Klang Valley¡±
  14. Infectious Diseases Forum 2017, Pullman Hotel, Kuala Lumpur. Invited speaker. Title: ¡°Antibiotics and sore throats: Major viruses and antibiotic resistance of bacteria isolated from primary care patients with acute pharyngitis (sore throat) in the Klang Valley¡±
  15. Genomic Applications of qPCR, dPCR & NGS 2016, National University of Singapore, Singapore. Invited speaker. Title: ¡°Quantitative real-time PCR of respiratory viruses in throat swabs: microfluidics vs nanofluidics¡±
  16. Infectious Diseases Forum 2016, Sunway Hotel, Kuala Lumpur. Invited speaker. Title: ¡°Of sore throats and normal throats: profiling of antibiotic-resistant bacteria in the Klang Valley¡±
  17. 5th Annual Next Generation Sequencing Asia Congress & 3rd Annual Single Cell Analysis Asia Congress 2015, A*STAR, Singapore. Invited speaker. Title: ¡°Gene expression in live cells - are all cells created equal?¡±

Awards and Recognitions

  1. Science Education Award (Runner-up Prize) 2021. Malaysia Toray Science Foundation (MTSF)
  2. MSMBB EduSTEMX 2020/2021 (as team leader). Malaysian Society for Molecular Biology & Biotechnology (MSMBB)
  3. MSMBB EduSTEMX 2020/2021 (as team mber). Malaysian Society for Molecular Biology & Biotechnology (MSMBB)
  4. Gold Medal - International Putra InnoCreative Poster Competition. International Putra Innocreative Carnival in Teaching & Learning 2020
  5. Science Education Award (Runner-up Prize) 2016. Malaysia Toray Science Foundation (MTSF)
  6. Ford Conservation & Environmental Grant 2014. Ford Motors Malaysia.
  7. John David Williams Memorial Award 2013. International Society of Chemotherapy.
  8. MAKNA Research Grant Award 2012. Malaysian Cancer Council-National Science Academy.
  9. Bronze Medal (first author): Invention, Research and Innovation Exhibition (PRPI) 2009, UPM.
  10. L¡¯Oreal for Women in Science National Fellowship 2006. L¡¯Oreal Malaysia-UNESCO
  11. Young Researcher Award (Molecular Biology) 2006. 15th Malaysian Society of Molecular Biology and Biotechnology (MSMBB) Scientific Meeting, Malaysia.
  12. Malaysian Patent PI 20097018 (2009) myIPO-Universiti Putra Malaysia. Compositions and Method of Cell Wall Disruption for Subsequent Preparation of Nucleic Acids
  13. Invited interviewee for IM4U Dre1m Fund 2017 on IM4U.fm, 10 Aug 2016.  
  14. Featured in Green is the Future (New Straits Times) 27th September 2015 Learning Curves pg1-3
  15. Mentioned in Research Success for Academician (The Star) 19th May 2015 StarSpecial pg12
  16. Mentioned in Improving Lives at ÎåÒ»³Ô¹Ï¹ÙÍø (New Straits Times) 15th August 2012 Supplement e3
  17. Mentioned in ÎåÒ»³Ô¹Ï¹ÙÍø Students Build Homes with Recycled Drink Packs (The Star Online - Nation) 30th April 2017 
  18. Appeared in an article featuring  ÎåÒ»³Ô¹Ï¹ÙÍø and Yayasan Generasi Gemilang (Nanyang Siang Pau) 12th May 2015 Supplement C3
  19. Appeared in an article featuring Health Awareness Campaign (China Press) 3rd May 2015 Central C10
  20. Appeared in an article featuring Organizing Health Awareness Campaign (Sin Chew Daily) 1st May 2015 Nation pg39 

Networks and Collaborations on Research

  1. For Women in Science Community online by L¡¯Or¨¦al-UNESCO For Women in Science Program
  2. Malaysia Institute of Medical Laboratory Sciences (MIMLS)- member
  3. Lifetime member - International Society of Infectious Diseases (ISID)

Contributions to Society

  1. Professors Without Borders (Prowibo) (2021-current) Volunteer professor to provide education content and access to underprivileged communities globally. Co-organize seminars, talks and activities benefitting the cause of inequality of access to quality education across the world.
  2. Volunteer Covid-19 real-time PCR lab specialist (2020 and 2021)
  3. ÎåÒ»³Ô¹Ï¹ÙÍø EcoCampus (2018-current). Initiator together with ÎåÒ»³Ô¹Ï¹ÙÍø Sustainable Development Goals (SDG) and World Wildlife Fund (WWF) and Foundation for Environmental Education (FEE).
  4. CAREton Project@ÎåÒ»³Ô¹Ï¹ÙÍø University aka CAREvolutin@ÎåÒ»³Ô¹Ï¹ÙÍø University (2013-current) Initiator and supervisor, ÎåÒ»³Ô¹Ï¹ÙÍø University-Tetra Pak Malaysia & Nestle Malaysia. Establishment and maintenance of a student-run collection and awareness project at the Faculty of Applied Sciences under the Tetra Pak-Nestle Malaysia CAREton Project umbrella that recovers used beverage cartons for upcycling into roofing tiles for underprivileged Orang Asli (Malaysian aborigine) homes.
  5. CAREton Project@ÎåÒ»³Ô¹Ï¹ÙÍø University. EPIC Homes Builder Volunteer, 12-14 December 2014, Kg Hulu Tamu Orang Asli (Indigenous People¡¯s Village), Hulu Selangor.
  6. CAREton Project@ÎåÒ»³Ô¹Ï¹ÙÍø University. EPIC Homes Builder Volunteer, 23-25 September 2016, Kg Orang Asli Serendah (Indigenous People¡¯s Village), Hulu Selangor.
  7. iM4U MyUniRocks! 2015 People¡¯s Choice Award (RM5,000 project grant) and RM5,000 project grant from IM4U Dre1M Fund 2017. Guided CAREton Project@ÎåÒ»³Ô¹Ï¹ÙÍø University to win these awards/grants as student team supervisor.
  8. CARE4Life Health Campaign (2014-2015) Technical advisor, ÎåÒ»³Ô¹Ï¹ÙÍø University (SAA)-iM4U. Advise and train FoAS and FHMS student volunteers on conducting free health screening tests (blood glucose, blood cholesterol, urine, blood pressure, body mass index) and health consultation for low-income communities.

Professional Affiliation

  1. International Society of Infectious Diseases (ISID) - member American Society of Microbiology (ASM) ¨C postdoctoral member
  2. Malaysian Society for M olecular Biology & Biotechnolog y (MSMBB) - member
  3. Golden Key International Honour Society ¨C member AIESEC UPM Chapter - member